Theo Bronkhorst made the remarks after his trial for failing to prevent an illegal hunt was postponed.
He said the case was "crazy" and the permits to kill Cecil the lion outside Hwange National Park had been obtained.
The killing in July has caused outrage around the world and the dentist, Walter Palmer, is in hiding.
Protests have been held outside his dental clinic in Minnesota and vandals spray-painted "lion killer" on the garage of his holiday home in Florida earlier this week.
The trial of Mr Bronkhorst was postponed on Wednesday to give his legal team more time to prepare their defence. If convicted, he faces up to 15 years in prison.
Mr Palmer is believed to have paid about $50,000 (£32,000) to hunt down Cecil, a major tourist attraction at Hwange National Park - Zimbabwe's largest game reserve.
Ms Muchinguri says Mr Palmer's extradition is being sought so that he can "be held accountable for his illegal action". The US is yet to respond to the request.